姓名

Yung-Fu Chang 張 永 富

學歷

DVM-1974 National Pingtung Institute of Agriculture, Taiwan (Veterinary Medicine)

M.S.-1981  University of Idaho, Moscow, Idaho

(Immunology/Pathology)

Ph.D. -1984 Texas A&M University, College Station, Texas

           (Microbiology/Immunology)

Postdoc-1984-1989 Texas A&M University,College Station, Texas(Molecular Biology)

經歷

1974-1979 Assistant Pathologist, Veterinary Pathology Division, Taiwan Provincial Research Institute for Animal Health, Taipei, Taiwan

1979-1981 Research Assistant and Graduate Student, Department of Veterinary Science, University of Idaho, Moscow, Idaho

1981-1984 Research Assistant and Graduate Student, Department of Veterinary Microbiology and Parasitology, College of Veterinary Medicine, Texas A&M University, College Station, Texas

1984-1985 Research Associate, Department of Medical Biochemistry and Genetics, College of Medicine, Texas A&M University, College Station, Texas

1986-1989 Assistant Research Scientist, Department of Medical Biochemistry and Genetics, College of Medicine, and Department of Biochemistry and Biophysics, College of Agriculture & Life Science, Texas A&M University, College Station, Texas

1989-1996 Assistant Professor and Assistant Director of the Microbiology Section, Diagnostic Center, College of Veterinary Medicine, Cornell University, Ithaca, New York

1996-2002 Associate Professor and Assistant Director of the Microbiology Section, Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York

2003      Visiting Professor, Department of Infectious disease and Medicine, College.

Jan–Jul   of Medicine, Stanford University. Stanford, California

2003-present Professor, Department of Population Medicine & Diagnostic Sciences, and Director of infectious disease Laboratory, New York State Animal Health Diagnostic Center, College of Veterinary Medicine, Cornell University, Ithaca, New York

現職

國立屏東科技大學 獸醫學院 講座教授(2011.8~迄今）

Professor of Molecular Microbiology, Director, Infectious Disease research

Laboratory, Animal Health Diagnostic Center

專長

個人網頁

曾獲得之

學術榮譽

通訊錄

地址

Department of Population Medicine and Diagnostic Sciences

C1-114, Vet Medical Center

College of Veterinary Medicine

Cornell University

Ithaca, New York 14853-5786

電話

傳真

E-Mail

yc42@cornell.edu

– Chang, Y. F., R. Young, and D. K. Struck. 1989. Cloning and characterization of a hemolysin gene from Actinobacillus (Haemophilus) pleuropneumoniae DNA &Cell biology: 8: 635-647.

– Cruz, W.T., R. Young, Y. F. Chang, and D.K. Struck. 1990. Deletion analysis resolves cell-binding and lytic domains of the Pasteurella leukotoxin. Mol. Microbiol. 4: 1933-1939.

– Chang, Y. F., R. Young, and D.K. Struck. 1991. The Actinobacillus pleuropneumoniae determinant:  unlinked appCA and appBD loci flanked by pseudogenes. J. Bacteriol. 173: 5151-5158.

– McWhinney, D.R., Y. F. Chang, R.Young, and D. K.Struck. 1992 Separable domains define target cell specificities of the RTX hemolysin from Actinobacillus pleuropneumoniae. J. Bacteriol. 174: 291-297.

– Appel, M. J.G., S. Allan, R. H. Jacobson, T.L. Lauderdale, Y. F. Chang, S. J. Shin, J. Thomford, R. Todhunter, and B. A. Summers. 1993.  Experimental Lyme disease in dogs produced arthritis and persistent infection. J. Infect. Dis. 167: 651-664.

– Chang, Y. F., D. P. Ma, J. Shi, and M. M. Chengappa. 1993.  Molecular characterization of a leukotoxin gene from a Pasteurella haemolytica-like organism, encoding a new member of RTX family. Infect. Immun. 61: 2089-2095.

– Chang, Y. F., J. Shi, D. P. Ma, S. J. Shin, and D. H. Lein. 1993. Molecular analysis of the Actinobacillus pleuropneumoniae RTX toxin-III gene cluster. DNA. Cell Biol. 12: 351-362.

– Frey, J., J. T. Bosse, Y. F. Chang, J.M. Cullen, B. Fenwick, G.F. Gerlach, D. Gygi, F. Haesebrouck, T. J. Inzana, R. Jansen, E. M. Kamp, J. Macdonald, J. I. MacInnes, K.R. Mittal, J. Nicolet, A.N. Rycroft, R.P.A.M. Segers, M.A. Smits, E. Stenbaek, D.K. Struck, J. F. Van Den Bosch, P. J. Wilson and R. Young.  1993.  Actinobacillus pleuropneumoniae RTX-toxins: Uniform designation of hemolysins, cytolysins, pleurotoxin and their genes. J. Gen. Microbiol. 139: 1723-1728.

– Chang, Y. F., M. J. Appel, R. H. Jacobson, S. J. Shin, P. Harpending, R. Straubinger, L. A. Patrican, H. Mohammed, and B. A. Summers. 1995. Recombinant OspA protects dogs against infection and disease caused by Borrelia burgdorferi. Infect. Immun. 63: 3543-3549.

– Straubinger, R. K., Y. F. Chang, R. H. Jacobson, and M. J. G. Appel. 1995.  Protection against Lyme disease: Sera from vaccinated dogs, but not from tick infected dogs, inhibit the in vitro growth of Borrelia burgdorferi. J. Clin. Microbiol. 33: 2745-2751.

– Chang, Y. F., R. Straubinger, R. H. Jacobson, J. B. Kim, T. J. Kim, D. Kim, S. J. Shin, and M. J. G. Appel. 1996. Dissemination of Borrelia burdorferi after experimental infection in dogs. J. Spiro. Tick-Borne Dis. 3: 80-86.

– Straubinger, R. K., A. F. Straubinger, L. Harter, R. H. Jacobson, Y. F. Chang, B. A. Summers, H. N. Erb, and M. J. G. Appel. 1997. Borrelia burgdorferi migration and proliferation causes up-regulation of interleukin-8 in synovial membranes of experimentally infected dogs. Infect. Immun. 65: 1273-1285.

– McDonough P. L., R. H. Jacobson, J. F. Timoney, A. Mutalib, D. C. Kradel, Y. F. Chang, S. J. Shin, D. H. Lein, S. Trock, and K. Wheeler. 1998. Interpretations of antibody responses to Salmonella enterica serotype Enteritidis gm flagellin in poultry flocks are enhanced by a Kinetics-based enzyme-linked immunosorbent assay. Clin. Diagn. Lab. Immunol. 5: 550-555.

– Chang, Y. F., V. Novosel, S. P. McDonough, R. H. Jacobson, C. F. Chang, T. Divers, F. W. Quimby, S. Shin, and D. H. Lein. 1999.  Vaccination against Lyme disease with recombinant Borrelia burgdorferi outer surface protein A (OspA) in horses. Vaccine 18: 540-548.

– Chang, Y. F.,S. P. McDonough, K.S. Shin, C. F. Chang, and T. Divers. 2000. Human granulocytic ehrlichiosis agent (HGE) infection in a pony vaccinated with recombinant OspA vaccine and challenged by exposure to naturally infected ticks. Clin. Diag. Lab. Immunol. 7: 68-71.

– Simpson, K.W., D. Strauss-Ayali, E. Scanziani, R. K. Straubinger, P. L. McDonough, A.F. Straubinger, Y. F. Chang, C. Domeneghini, N. Arebi, and J. Calm. 2000.  Helicobacter felis infection is associated with lymphpoid follicular hyperplasia and mild gastritis but normal gastric secretory function in cats. Infect. Immun. 68: 779-790.

– Simpson, K.W., D. Strauss-Ayali, E. Scanziani, R. K. Straubinger, P. L. McDonough, A. F. Straubinger, Y. F. Chang, M. Esteves, J. G. Fox, C. Domeneghini, N. Arebi, and J. Calam. 2001.  Gastric secretory function in cats with Helicobacter pylori infection. Helicobacter 6: 1-14.

– Raghavan, P. U. M., Y. F. Chang, S. S. D. Jusuf, S. Artiushin, J. F. Timoney, S. P. McDonough, S. C. Barr, T. J. Divers, P. McDonough, K. W. Simpson, and H. Mohammed. 2002. Cloning and molecular characterization of an immunogenic LigA of Leptospira interrogans. Infect. Immun. 70:5924-5930.

– Teng, C.H., R. U.M. Palaniappan, and Y. F. Chang. 2003. Cloning and characterization of an Ehrlichia canis gene encoding a protein localized to the morula membrane. Infect. Immun. 71:2218-2225. .

– Palaniappan, R. U., Y. F. Chang, F. Hassan, S.P. McDonough, M. Pough, S.C. Barr, K. W. Simpson, H. O. Mohammed, S. Shin, P. McDonough, R.  Zuerner, J. Qu,  & B. Roe. 2004. Expression of leptospiral immunoglobulin-like protein from Leptospiral interrogans and evaluation of its diagnostic potential in kinetic enzyme linked immunosorbent assay. J. Med. Microbiol. 53: 975-84.

– Wang, Z., Z. Yuan, M. Matsumoto, U. R. Hengge and Y. F. Chang. 2005. Immune responses with DNA vaccines encoded different gene fragments of severe acute respiratory syndrome coronavirus in BALB/c mice. BioChem. BioPhy. Res. Com. 327:130-135.

– Shin, S.J., C.F. Chang, C. C. Chang, S. P. McDonough, B. Thompson, H.S. Yoo, and Y. F. Chang. 2005. In vitro cellular immune response to recombinant antigens of Mycobacterium avium subsp. paratuberculosis. Infect. Immun. 73:5074-5085.

– Ku, Y.W., S. P. McDonough, R.U.M. Palaniappan, C. F. Chang, Y. F. Chang. 2005. Identification and characterization of in vivo attenuated mutants of Salmonella enterica serovar Choleraesuis using signature-tagged mutagenesis in a pig infection model. Infect. Immun. 73:8194-8203.

– Raghavan, P. U. M., S. P. McDonough, T. J. Divers, C. S. Chen, M.J. Pan, M. Matsumoto and Y. F. Chang. 2006. Immunoprotection of recombinant leptospiral immunoglobulin like A protein (LigA) against Leptospira interrogans serovar Pomona infection. Infect. Immun. 74:1745-1750.

– Palaniappan, R. U., Y. Zhang, D. Chiu, A. Torres, C. DebRoy, T. S. Whittam and Y. F. Chang. 006. Differentiation of Escherichia coli pathotypes by oligonucleotide spotted array. J. Clin. Microbiol. 44:1495-1501.

– Palaniappan, R. U., S. Ramanujam and Y. F. Chang. 2007. Leptospirosis: Pathogenesis, Immunity, and Diagnosis. Current Opinion Infect. Dis. 20:284-292.

– Lin, Y.P. and Y. F. Chang. 2007. A domain of the Leptospira LigB contributes to high binding of fibronectin. Biochem. Biophy. Res. Comm. 362:443-448.

– Chang, Y.F., C. S. Chen, R. U.M. Palaniappan, S. P. McDonough, W. Yang, M. J. Pan, and C. F. Chang. 2007. Immunogenicity and protection of the recombinant leptospiral immunogenic proteins as vaccine candidates. Vaccine 25: 8190-8197.

– Faisal, S.M., W. Yan, C. S. Chen, R. U.M. Palaniappan, S. P. McDonough, and Y. F. Chang. 2008. Evaluation of protective immunity of Leptospira immunoglobulin like protein A (LigA) DNA vaccine against challenge in hamsters. Vaccine, 26:277-287.

– Chen, L. H., K. Kathaperumal, C. J. Huang, S. P. McDonough, S. Stehman, B. Akey, J. Huntley, C.F. Chang and Y. F. Chang. 2008. Immune responses in mice to Mycobacterium avium subsp. paratuberculosis following vaccination with a novel 74F recombinant polyprotien. Vaccine 26:1253-1262.

– Kathaperumal, K., S. U. Park, S. P. McDonough, S. Stehman, B. Akey, J. Huntley, S. Wong, L. H. Chen, C.-F. Chang and Y. F. Chang. 2008. Vaccination with recombinant Mycobacterium avium subsp. paratuberculosis proteins induces differential immune responses and protects calves against infection by oral challenge. Vaccine, 26:1652-1663. .

– Scaria, S., P. U. M. Palaniappan, D. Chiu, J.A. Phan, L. Ponnala, P. McDonough, Y. T. Grohn, S. Porwollik, M. McClelland, C.S. Chiou, C. Chu and Y. F. Chang. 2008. Microarray for molecular typing of Salmonella enterica serovars. Mol. Cell. Probes 22:238-243.

– Park, S.U., K. Kathaperumal, S. McDonough, B. Akey, J. Huntley, J. P. Bannantine and Y.F. Chang. 2008.  Immunization with a DNA vaccine cocktail induces a Th1 response and protects mice against Mycobacterium avium subsp. paratuberculosis. Vaccine. 26:4329-4337.

– Lin, Y.P. R. Raman, Y. Sharma, and Y. F. Chang. 2008. Calcium binds to Leptospiral immunoglobulin-like protein, LigB and modulates fibronectin-binding. J. Biol. Chem. 283(12): 25140-25149.

– Scaria, J., A. Sreedharan, and Y. F. Chang. 2008. Microbial Diagnostic Array Workstation (MDAW): A web server for diagnostic array data storage, sharing and analysis. Source Code Biol. Med. 3:14-18.

– Kathaperumal, K,, V. Kumanan, S. McDonough, L. H. Chen, S. U. Park, M. A. Moreira, B. Akey, J. Huntley, C. F. Chang, and Y. F. Chang 2009. Evaluation of immune responses and protective efficacy in a goat model following immunization with a coctail of recombinant antigens and a polyprotein of Mycobacterium avium subsp. paratuberculosis. Vaccine 27:123-135.

– Faisal, S.M., W. Yan, S. P. McDonough, and Y. F. Chang 2009. Leptospira immunoglobulin-like protein A variable region (LigAvar) incorporated in Liposomes and PLGA-Microspheres produces a robust immune response correlating to protective immunity. Vaccine 27:378-387.

– Yan, W., S. M. Faisal, S. P. McDonough, T. J. Divers, S. C. Barr, M. J. Pan, C. F. Chang and Y.F. Chang. 2009. Recombinant Leptospira Immunoglobulin-Like Protein B (rLigB) protects hamsters against lethal Leptospira interrogans infection. Microbes Infect.11:230-237.

– Janvilisri T., J. Scaria, A. Thompson, A. Nicholson, B. Limbargo, L. G. Arroyo, J. G. Songer, Y. T. Grohn, and Y. F. Chang. 2009. Microarray identification of Clostridium difficile core components and divergent regions associated with host origin. J. Bacteriol. 191:3881-3891.

– Lin Y.P., D.W. Lee, S. P. McDonough, L. K. Nicholson, Y. Sharma, and Y. F. Chang.2009. Repeated domains of leptospira immunoglobulin-like proteins interact with elastin and tropoelastin. J. Biol. Chem. 284:19380-19391.

– Lin Y.P., A. Greenwood, L. K. Nickolson, Y. Sharma, S. P.McDonough, and Y. F. Chang. 2009. Fibronectin binds to and induces conformational change in a disordered region of Leptospiral immunoglobulin-like protein B. J. Biol. Chem. 284:23547-23557.

– Janvilisri T., J. Scaria, R. Gleed, S. Fubini, M. M. Bonkosky, Y. T. Grohn, and Y. F. Chang. 2010. Development of a microarray for identification of pathogenic Clostridium species. Diagn. Microbiol. Infect. Dis. 66:140-147.

– Yan, W., S. M. Faisal, S. P. McDonough, C. F. Chang, M. J. Pan, and Y.F. Chang. 2010. Identification and characterization of OmpA-like proteins as novel vaccine candidates for Leptospirosis. Vaccine.  28:2277-2283.

– Janvilisri T., J. Scaria, and Y. F. Chang. 2010. Transcriptional profiling of Clostridium difficile and Caco-2 cells during infection.  J. Infect. Dis. 202:282-290.

– Lin, Y.P., S. P.McDonough., Y Sharma, and Y. F. Chang. 2010. The terminal Immunoglobulin-like repeats of LigA and LigB of Leptospira enhance their binding to gelatin binding domain of fibronectin and host cell. PLOS ONE. 5:e11301.

– Davis, M.A., J.Y.Lim, Y. Soyer, H. Harbottle,Y. F. Chang , D. New, L. H.Orfe, T. E. Besser, and D. R. Call Development and validation of a resistance and virulence gene microarray targeting Escherichia coli and Salmonella enterica. J Microbiol Methods. 2010 82:36-41.

– Scaria, J., L.D Warnick, J. B. Kaneene, K. May, C. H. Teng and Y. F. Chang^. 2010. Comparison of phenotypic and genotypic antimicrobial profiles in Eschericia coli and Salmonella enterica from the same dairy cattle farms. Mole. Cell. Probes. 24:325-45.

– Scaria, J., L. Ponnala, T. Janvilisri, W. Yan, L. A. Mueller and Y. F. Chang. 2010. Analysis of Ultra Low Genome Conservation in Clostridium difficile. PLoS One.  5: e15147.

– Rama, R., V. Rajanikanth, R. U. M. Palaniappan, Y.P. Lin, H. He, S. P. McDonough, Y. Sharma and Y. F. Chang. 2010. Big Domains are Novel Ca²⁺-binding Modules: Evidences from Big Domains of Leptospira immunoglobulin-like (Lig) Proteins. PLoS One. e14377.

– Rajkhowa^, S., J. Scaria, D. L Garcia, K. A. Musser, B. L. Akey, and Y. F. Chang. 2010. Analysis of Escherichia coli O157 clinical isolates by multilocus sequence typing. BMC Research Notes. 3:343.

– Lin, Y.P., C. J. Kuo, X. Koleci, S. P. McDonough, and Y. F. Chang. 2011. Manganese binds to Clostrdium difficile Fbp68 and is essential for fibronectin binding. J.  Biol. Chem. 286:3957-3969, 2011.

– Lin, Y.P., S. P. McDonough, Y. Sharma, and Y. F. Chang. 2011. Leptospiral immunoglobulin-like protein (LigB) binds to the C-teminal fibrinogen αC domain inhibiting fibrin clot formation, platelet adhesion, and aggregation. Mol. Microbiol. 79:1063-1076.

– Faisal S.M, J. W. Chen, S.P. McDonough, C.F. Chang, C.H. Teng, and Y.F. Chang. 2011. Immunostimulatory and antigen delivery properties of liposomes made up of total polar lipids from non-pathogenic bacteria leads to efficient induction of both innate and adaptive immune responses. Vaccine 29:2381-2391.

– Scaria J., T. Janvilisri, S. Fubini, R. D. Gleed, S. P. McDonough, and Y.F. Chang. 2011. Clostridium difficile transcriptome analysis using pig ligated loop model reveals modulation of pathways not modulated in vitro. J. Infect. Dis. 203:1613-1620.

– Cao Y, S.M. Faisal,W. Yan, Y.C. Chang,S. P. McDonough,N. Zhang, B. L.Akey, and Y. F. Chang. 2011. Evaluation of novel fusion proteins derived from extracellular matrix binding domains of LigB as vaccine candidates against leptospirosis in a hamster model. Vaccine. 29:7379-86

– Chen J.W, S. M.Faisal, S. Chandra, S. P. McDonough, M. A. Moreira, J. Scaria, C. F. Chang, J. P. Bannantine, B. Akey B, and Y. F. Chang. 2012. Immunogenicity and protective efficacy of the Mycobacterium avium subsp. paratuberculosis attenuated mutants against challenge in a mouse model. Vaccine. [Epub ahead of print]

– Kuo C.J, H. Bell, C. L. Hsieh, C. P. Ptak, and Y. F. Chang. 2012.  The novel Mycobacteria antigen 85 complex binding motif on Fibronectin. J Biol Chem. 287:1892-1902.

 (B) 專書及專書章節(Proceedings, Monograph or Book Chapter)

– Divers, T.J., T. S. Mair, and Y. F. Chang. 2009. Lyme disease in horses. pp286-292. In T. S. Mair and R.E. Hutchinson (ed). Infectious diseases of the horses. Geering Print Ltd. Ashford, Kent, UK.

– Divers, T.J. and Y. F. Chang. 2009. Lyme disease. pp143-144. In N. Edward Robinson and Kim A. Sprayberry (ED). Current therapy in Equine Medicine. Saunders, St. Louis, Missouri.

– Divers, T. J. and Y. F. Chang. 2009. Leptospirosis. pp145-147. In N. Edward Robinson and Kim A. Sprayberry (ED). Current therapy in Equine Medicine. Saunders, St. Louis, Missouri.

– Bannantine, J.P. Y. F. Chang, and V. Kapur. 2010. Chapter 15. Mycobacterium avium subsp. paratuberculosis. Book title: Genomics of Foodborne and Waterborne Pathogens, ASM, ISBN 978-1-84593-613-6

– Faisal S. M., S. P. McDonough, and Y. F. Chang. 2012. Chapter 7.  Leptospira: Invasion, Persistence and Pathogenesis. Book Title: The Pathogenic Spirochetes: strategies for evasion of host immunity and persistence.  Springer Science, in Press.